ESTUDIO DE LA MORFOLOGÍA Y ACLIMATACIÓN DE PLANTAS DE LAELIA EYERMANIANA RCHB. f. GENERADAS IN VITRO

Abstract

A morphological study of plantlets of Laelia
eyermaniana Rchb.f. cultured in vitro was
carried out. Histologic preparations were
made to observe different stages from seed
germination until ex vitro acclimatization
of plantlets. Photomicrographs showed
various transitional stages. Seven relative
stages of development were identified
and described: seed, non-photosynthetic
protocorm, photosynthetic protocorm,
differentiated protocorm, plantlets with
leafs, plantlets with leaves and roots, and
plantlets with leaves and two roots, as
well as two transitory phases: somatic
embryogenic mass (SEM) and protocormlike bodies (PLBs) obtained by indirect
somatic embryogenesis. Stomatal leaf
density doubled under ex vitro conditions.
This study contributes to the knowledge
of morphological development of L.
eyermaniana during in vitro culture and ex
vitro acclimatization.