Growth of Laelia autumnalis and Encyclia cordigera seedlings as a function of sucrose and activated charcoal concentration
DOI:
https://doi.org/10.18387/polibotanica.61.13Keywords:
orchids, carbohydrates, growth.Abstract
In vitro culture uses different components to improve plant growth; among them, the addition of different concentrations of sucrose and activated charcoal to the culture media has favored the growth of many orchid species. The objective of the research was to evaluate the effect of three concentrations of sucrose and activated charcoal on the in vitro culture of seedlings Laelia autumnalis and Encyclia cordigera was evaluated to determine the most suitable for growth in each species. The macro and micronutrients of the Murashige and Skooh (1962) medium were used, supplemented with 0.5 mg L-1 of thiamine HCI, 100 mg L-1 of myoinositol and three concentrations of sucrose (15, 30 and 45 g L-1) and activated charcoal (0, 1 and 3 g L-1). A completely randomized experimental design with ten repetitions was used. In L. autumnalis, 45 g of sucrose and 1 g of activated charcoal generated the best results for plant height (31.97 mm), leaf length (13.44 mm) and roots (8.63 cm), plant and root dry matter (6.57 and 2.65 mg, respectively). Similarly In E. cordigera, 45 g of sucrose and 3 g of activated charcoal promoted the plant highest (6.06 mm), number (5.78) and length of leaf (21.54 mm), number of roots (8.02), protocorm diameter (3.21 mm), plant and roots dry matter (27.78 and 52.92 mg, respectively). The plants were smaller in media without activated charcoal.
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