In vitro conservation of Guarianthe skinneri (Bateman) Dressler & W. E. Higgins for minimal growth
DOI:
https://doi.org/10.18387/polibotanica.58.12Keywords:
Conservation, Minimum growth, Ancimidol, Polyethyleneglycol, In vitro regenerationAbstract
In vitro conservation techniques are considered methods of medium-term conservation of plant germplasm. The conservation period under this technique is usually 1 to 2 years without using any subculture. In Mexico there are plant genetic resources that are cataloged within the Official Mexican Standard NOM-059-SEMARNAT-2010. These plant species must be safeguarded and conserved as a priority. Guarianthe skinneri (Bateman) Dressler & W. E. Higgins is an orchid that has a flower that attracts the attention of horticulturists and/or collectors as an ornamental plant. The objective of this study was to establish an in vitro conservation protocol for G. skinneri through minimal growth. For this, different concentrations (0, 0.5, 1.0 and 2.0 mg L-1) of abscisic acid (ABA), paclobutrazol (PBZ), ancymidol (ACD) and different concentrations (0, 5, 10 and 20 g L-1) of polyethylene glycol (8000). Subsequently, the regeneration of the preserved material was carried out using MS medium added with 2 mg L-1 of 6-benzylaminopurine (BAP). After 12 months of in vitro conservation, the results showed a reduction in the length of the shoots preserved in MS with 2.0 mg L-1 of PBZ and in ABA 0.5 mg L-1. However, in the PBZ treatment, shoot and root formation increased compared to the ABA treatment. The regeneration of 5.4 shoots/explant was achieved at 2.0 mg L-1 of BAP. As well as a survival of 90% during the acclimatization process. The results of this study can contribute to the conservation work carried out on this species of ornamental importance.
References
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Chen, K., Li, G. J., Bressan, R. A., Song, C. P., Zhu, J. K., & Zhao, Y. (2020). Abscisic acid dynamics, signaling, and functions in plants. Journal of integrative plant biology, 62(1), 25-54. https://doi.org/10.1111/jipb.12899
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Cruz-Cruz, C. A., González-Arnao, M. T., Bautista-Aguilar, J. R., & Ramírez-Mosqueda, M. A. (2022). In vitro short-term storage of Stanhopea tigrina Bateman ex Lind. South African Journal of Botany, 151, 334-338. https://doi.org/10.1016/j.sajb.2022.10.014
Desta, B., & Amare, G. (2021). Paclobutrazol as a plant growth regulator. Chemical and Biological Technologies in Agriculture, 8(1), 1-15. https://doi.org/10.1186/s40538-020-00199-z
Gabriela, M. L. N., & Yaayé, A. C. (2015). El papel de la comercialización orquídeas y bromelias de mercados de los valles centrales de Oaxaca, México, en la subsistencia campesina. Observatorio de la Economía Latinoamericana, (210). https://ideas.repec.org/a/erv/observ/y2015i21031.html
Gutiérrez–Rodríguez, B. E. (2022). The importance of forests in the conservation and prevalence of orchids in Megamexico. Agroproductividad, 15(5). https://doi.org/10.32854/agrop.v15i5.2187
Hernández-Ramírez, F., Iracheta-Donjuan, L., Damon, A. A., Fernández-Pavía, S. P., & Guillén-Navarro, K. (2023). Efecto del medio de cultivo y escotoperiodo en la germinación de semillas y crecimiento in vitro de Guarianthe skinneri (Bateman) Dressler & WE Higgins (Orchidaceae). Polibotánica, (56), 151-170. https://doi.org/10.18387/polibotanica.56.8
Kamińska, M., Kęsy, J., & Trejgell, A. (2021). Abscisic acid in preservation of Taraxacum pieninicum in the form of synthetic seeds in slow growth conditions. Plant Cell, Tissue and Organ Culture, 144, 295-312. https://doi.org/10.1007/s11240-020-01924-0
Leyva-Ovalle, O. R., Bello-Bello, J. J., Murguía-González, J., Núñez-Pastrana, R., & Ramírez-Mosqueda, M. A. (2020). Micropropagation of Guarianthe skinneri (Bateman) Dressler et WE Higging in temporary immersion systems. 3 Biotech, 10, 1-8. https://doi.org/10.1007/s13205-019-2010-3
López Puc, G., & Herrera-Cool, G. J. (2022). Asymbiotic germination, in vitro conservation and regeneration of Catasetum integerrimum Hook. Polibotánica, (53), 135-149. https://doi.org/10.18387/polibotanica.53.9
Mackenzie, S., & Yates, D. (2016). Collectors on illicit collecting: Higher loyalties and other techniques of neutralization in the unlawful collecting of rare and precious orchids and antiquities. Theoretical Criminology, 20(3), 340-357. https://doi.org/10.1177/1362480615607625
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nausch, H., & Buyel, J. F. (2021). Cryopreservation of plant cell cultures–Diverse practices and protocols. New Biotechnology, 62, 86-95. https://doi.org/10.1016/j.nbt.2021.02.002
Orozco-Meléndez, L. R., Hernández-Rodríguez, O. A., Cruz-Álvarez, O., Robles-Hernández, L., Ávila-Quezada, G. D., Chavez, E. S., Porras-Flores, D. A. & Ojeda-Barrios, D. L. (2022). Paclobutrazol and its use in fruit production: A review. Phyton, 91(1), 1. https://doi.org/10.32604/phyton.2022.016908
Ramírez-Mosqueda, M. A., Cruz-Cruz, C. A., Atlahua-Temoxtle, J., & Bello-Bello, J. J. (2019). In vitro conservation and regeneration of Laelia anceps Lindl. South African Journal of Botany, 121, 219-223. https://doi.org/10.1016/j.sajb.2018.11.010
Rathour, R., Yadav, S., Singh, A., Kaushik, S., & Rai, M. K. (2023). A liquid culture system for plantlet conversion and slow growth storage of encapsulated shoot tips of Justicia adhatoda L. Industrial Crops and Products, 205, 117534. https://doi.org/10.1016/j.indcrop.2023.117534
Rodríguez, L. A. B., Rivera, B. M., & Maya, A. P. (2012). Etnoecología de la flor de catarina-Laelia autumnalis (La Llave & Lex.) Lindl.)-(Orchidaceae) en una comunidad campesina al sur del estado de Morelos, México: conservando un recurso y preservando saberes populares. Etnobiología, 10(1), 1-17. https://dialnet.unirioja.es/servlet/articulo?codigo=5294459
Sahu, P. K., Sao, R., Khute, I. K., Baghel, S., Patel, R. R. S., Thada, A., ... & Sharma, D. (2023). Plant Genetic Resources: Conservation, Evaluation and Utilization in Plant Breeding. En: Advanced Crop Improvement, Volume 2: Case Studies of Economically Important Crops, Raina, A., Wani, M. R., Laskar, R. A., Tomlekova, N., Khan, S., (eds.) (pp. 1-45). Cham: Springer International Publishing. https://doi.org/10.1007/978-3-031-26669-0_1
Salgotra, R. K., & Chauhan, B. S. (2023). Genetic diversity, conservation, and utilization of plant genetic resources. Genes, 14(1), 174. https://doi.org/10.3390/genes14010174
Samarina, L. S., Choudhary, R., Kolomiets, T. M., Abilfazova, Y. S., & Saran, P. L. (2014). In vitro Conservation Technique for Russian Citrus limon. Agricultural Research, 3, 279-283. https://doi.org/10.1007/s40003-014-0127-5
SEMARNAT (2010) NOM-059-SEMARNAT-2010, Protección ambiental Especies nativas de México de flora y fauna silvestres-Categorías de riesgo y especificaciones para su inclusión, exclusión o cambio-Lista de especies en riesgo. SEMARNAT, CDMX. https://www.dof.gob.mx/nota_detalle.php?codigo=5578808&fecha=14/11/2019#gsc.tab=0
Sharma, N., Pandey, R., & Agrawal, A. (2020). Influence of explant types, non-embryogenic synseed and reduced oxygen environment on in vitro conservation of Bacopa monnieri (L.) Wettst. In Vitro Cellular & Developmental Biology-Plant, 56(6), 851-856. https://doi.org/10.1007/s11627-020-10078-9
Sosa-Nishizaki, Ó. (2009). Impacto de los factores antropogénicos de afectación directa a las poblaciones silvestres de flora y fauna. En: Capital natural de México, Soberón J., Halffter G., Llorente-Bousquets J., (eds.). pp. 247-276. https://biblioteca.ecosur.mx/cgi-bin/koha/opac-detail.pl?biblionumber=000037477
Spinoso-Castillo, J. L., Pérez-Sato, J. A., Schettino-Salomón, S. S., & Bello-Bello, J. J. (2022). An alternative method for medium-term in vitro conservation of different plant species through gibberellin inhibitors. In Vitro Cellular & Developmental Biology-Plant, 58(4), 606-614. https://doi.org/10.1007/s11627-022-10263-y
Al-Abdallat, A. M., Shibli, R. A., Akash, M. W., Rabbaa, M., & Al-Qudah, T. (2017). In vitro preservation of transgenic tomato (Solanum lycopersicum L.) plants overexpressing the stress-related SlAREB1 transcription factor. International Journal of Molecular Sciences, 18(7), 1477. https://doi.org/10.3390/ijms18071477
Bautista-Aguilar, J. R., Iglesias-Andreu, L. G., Martínez-Castillo, J., Ramírez-Mosqueda, M. A., & Ortiz-García, M. M. (2021). In Vitro Conservation and Genetic Stability in Vanilla planifolia Jacks. HortScience, 56(12), 1494-1498. https://doi.org/10.21273/HORTSCI16118-21
Benelli, C., Tarraf, W., Izgu, T., & De Carlo, A. (2022). In Vitro Conservation through Slow Growth Storage Technique of Fruit Species: An Overview of the Last 10 Years. Plants, 11(23), 3188. https://doi.org/10.3390/plants11233188
Bertolini V, Damon A, Ibarra-Cerdeña C (2016) Atlas de las orquídeas del Soconusco: modelos digitales de nichos ambientales entre Centro y Sudamérica. El Colegio de la Frontera Sur, Tapachula, pp 56. https://www.researchgate.net/publication/306465164
Bettoni, J. C., Bonnart, R., & Volk, G. M. (2021). Challenges in implementing plant shoot tip cryopreservation technologies. Plant Cell, Tissue and Organ Culture, 144(1), 21-34. https://doi.org/10.1007/s11240-020-01846-x
Castillo-Pérez, L. J., Martínez-Soto, D., Maldonado-Miranda, J. J., Alonso-Castro, A. J., & Carranza-Álvarez, C. (2019). The endemic orchids of Mexico: a review. Biologia, 74, 1-13. https://doi.org/10.2478/s11756-018-0147-x
Chandran, S., Raghu, A. V., & Mohanan, K. V. (2023). In vitro conservation of rare, endangered, and threatened plants. En: Conservation and Sustainable Utilization of Bioresources, Sukumaran, S. T., Keerthi, T. R. (pp. 391-408). Singapore: Springer Nature Singapore. https://doi.org/10.1007/978-981-19-5841-0_16
Chauhan, R., Singh, V., & Quraishi, A. (2019). In vitro conservation through slow-growth storage. Synthetic Seeds: En: Germplasm Regeneration, Preservation and Prospects, Faisal, M., Alatar, A. A. (eds.) 397-416. https://doi.org/10.1007/978-3-030-24631-0_19
Chen, K., Li, G. J., Bressan, R. A., Song, C. P., Zhu, J. K., & Zhao, Y. (2020). Abscisic acid dynamics, signaling, and functions in plants. Journal of integrative plant biology, 62(1), 25-54. https://doi.org/10.1111/jipb.12899
CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora). 2012-2023. Appendices I, II and III. CITES, Geneva, Switzerland. Available from http://www.cites.org/eng/app/appendices.php (accessed August 2023).
Coutiño-Cortés, A. G., Bertolini, V., Morales, F. A., Valle-Mora, J., Iracheta-Donjuan, L., García-Bautista, M., & Ruiz-Montoya, L. (2018). El uso ornamental de Guarianthe skinneri (Orchidaceae), en Chiapas y Guatemala, determina parcialmente su diversidad y estructura genética. Acta botánica mexicana, (124), 35–48. https://doi.org/10.21829/abm12 4.2018.1303
Cruz-Cruz, C. A., González-Arnao, M. T., Bautista-Aguilar, J. R., & Ramírez-Mosqueda, M. A. (2022). In vitro short-term storage of Stanhopea tigrina Bateman ex Lind. South African Journal of Botany, 151, 334-338. https://doi.org/10.1016/j.sajb.2022.10.014
Desta, B., & Amare, G. (2021). Paclobutrazol as a plant growth regulator. Chemical and Biological Technologies in Agriculture, 8(1), 1-15. https://doi.org/10.1186/s40538-020-00199-z
Gabriela, M. L. N., & Yaayé, A. C. (2015). El papel de la comercialización orquídeas y bromelias de mercados de los valles centrales de Oaxaca, México, en la subsistencia campesina. Observatorio de la Economía Latinoamericana, (210). https://ideas.repec.org/a/erv/observ/y2015i21031.html
Gutiérrez–Rodríguez, B. E. (2022). The importance of forests in the conservation and prevalence of orchids in Megamexico. Agroproductividad, 15(5). https://doi.org/10.32854/agrop.v15i5.2187
Hernández-Ramírez, F., Iracheta-Donjuan, L., Damon, A. A., Fernández-Pavía, S. P., & Guillén-Navarro, K. (2023). Efecto del medio de cultivo y escotoperiodo en la germinación de semillas y crecimiento in vitro de Guarianthe skinneri (Bateman) Dressler & WE Higgins (Orchidaceae). Polibotánica, (56), 151-170. https://doi.org/10.18387/polibotanica.56.8
Kamińska, M., Kęsy, J., & Trejgell, A. (2021). Abscisic acid in preservation of Taraxacum pieninicum in the form of synthetic seeds in slow growth conditions. Plant Cell, Tissue and Organ Culture, 144, 295-312. https://doi.org/10.1007/s11240-020-01924-0
Leyva-Ovalle, O. R., Bello-Bello, J. J., Murguía-González, J., Núñez-Pastrana, R., & Ramírez-Mosqueda, M. A. (2020). Micropropagation of Guarianthe skinneri (Bateman) Dressler et WE Higging in temporary immersion systems. 3 Biotech, 10, 1-8. https://doi.org/10.1007/s13205-019-2010-3
López Puc, G., & Herrera-Cool, G. J. (2022). Asymbiotic germination, in vitro conservation and regeneration of Catasetum integerrimum Hook. Polibotánica, (53), 135-149. https://doi.org/10.18387/polibotanica.53.9
Mackenzie, S., & Yates, D. (2016). Collectors on illicit collecting: Higher loyalties and other techniques of neutralization in the unlawful collecting of rare and precious orchids and antiquities. Theoretical Criminology, 20(3), 340-357. https://doi.org/10.1177/1362480615607625
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nausch, H., & Buyel, J. F. (2021). Cryopreservation of plant cell cultures–Diverse practices and protocols. New Biotechnology, 62, 86-95. https://doi.org/10.1016/j.nbt.2021.02.002
Orozco-Meléndez, L. R., Hernández-Rodríguez, O. A., Cruz-Álvarez, O., Robles-Hernández, L., Ávila-Quezada, G. D., Chavez, E. S., Porras-Flores, D. A. & Ojeda-Barrios, D. L. (2022). Paclobutrazol and its use in fruit production: A review. Phyton, 91(1), 1. https://doi.org/10.32604/phyton.2022.016908
Ramírez-Mosqueda, M. A., Cruz-Cruz, C. A., Atlahua-Temoxtle, J., & Bello-Bello, J. J. (2019). In vitro conservation and regeneration of Laelia anceps Lindl. South African Journal of Botany, 121, 219-223. https://doi.org/10.1016/j.sajb.2018.11.010
Rathour, R., Yadav, S., Singh, A., Kaushik, S., & Rai, M. K. (2023). A liquid culture system for plantlet conversion and slow growth storage of encapsulated shoot tips of Justicia adhatoda L. Industrial Crops and Products, 205, 117534. https://doi.org/10.1016/j.indcrop.2023.117534
Rodríguez, L. A. B., Rivera, B. M., & Maya, A. P. (2012). Etnoecología de la flor de catarina-Laelia autumnalis (La Llave & Lex.) Lindl.)-(Orchidaceae) en una comunidad campesina al sur del estado de Morelos, México: conservando un recurso y preservando saberes populares. Etnobiología, 10(1), 1-17. https://dialnet.unirioja.es/servlet/articulo?codigo=5294459
Sahu, P. K., Sao, R., Khute, I. K., Baghel, S., Patel, R. R. S., Thada, A., ... & Sharma, D. (2023). Plant Genetic Resources: Conservation, Evaluation and Utilization in Plant Breeding. En: Advanced Crop Improvement, Volume 2: Case Studies of Economically Important Crops, Raina, A., Wani, M. R., Laskar, R. A., Tomlekova, N., Khan, S., (eds.) (pp. 1-45). Cham: Springer International Publishing. https://doi.org/10.1007/978-3-031-26669-0_1
Salgotra, R. K., & Chauhan, B. S. (2023). Genetic diversity, conservation, and utilization of plant genetic resources. Genes, 14(1), 174. https://doi.org/10.3390/genes14010174
Samarina, L. S., Choudhary, R., Kolomiets, T. M., Abilfazova, Y. S., & Saran, P. L. (2014). In vitro Conservation Technique for Russian Citrus limon. Agricultural Research, 3, 279-283. https://doi.org/10.1007/s40003-014-0127-5
SEMARNAT (2010) NOM-059-SEMARNAT-2010, Protección ambiental Especies nativas de México de flora y fauna silvestres-Categorías de riesgo y especificaciones para su inclusión, exclusión o cambio-Lista de especies en riesgo. SEMARNAT, CDMX. https://www.dof.gob.mx/nota_detalle.php?codigo=5578808&fecha=14/11/2019#gsc.tab=0
Sharma, N., Pandey, R., & Agrawal, A. (2020). Influence of explant types, non-embryogenic synseed and reduced oxygen environment on in vitro conservation of Bacopa monnieri (L.) Wettst. In Vitro Cellular & Developmental Biology-Plant, 56(6), 851-856. https://doi.org/10.1007/s11627-020-10078-9
Sosa-Nishizaki, Ó. (2009). Impacto de los factores antropogénicos de afectación directa a las poblaciones silvestres de flora y fauna. En: Capital natural de México, Soberón J., Halffter G., Llorente-Bousquets J., (eds.). pp. 247-276. https://biblioteca.ecosur.mx/cgi-bin/koha/opac-detail.pl?biblionumber=000037477
Spinoso-Castillo, J. L., Pérez-Sato, J. A., Schettino-Salomón, S. S., & Bello-Bello, J. J. (2022). An alternative method for medium-term in vitro conservation of different plant species through gibberellin inhibitors. In Vitro Cellular & Developmental Biology-Plant, 58(4), 606-614. https://doi.org/10.1007/s11627-022-10263-y
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2024-06-21
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In vitro conservation of Guarianthe skinneri (Bateman) Dressler & W. E. Higgins for minimal growth. (2024). POLIBOTANICA, 58. https://doi.org/10.18387/polibotanica.58.12
